PrimePCR™ Probe Assay: Pou4f1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047782
Assay Design:   Exonic
Chromosome Location:   15:92117120-92117268question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000012828

Gene Information

transcription factor containing the Pit1-Oct1-Unc86 (POU) domain; regulates expression of Trk receptors and Bcl-2 family members; associated with sensory neuron survival [RGD, Feb 2006]

Gene Symbol:   Pou4f1
Gene Name:   POU domain, class 4, transcription factor 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000009669
Entrez:   114503
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 37.720000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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