PrimePCR™ Probe Assay: Cdc42bpa, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023090
Assay Design:   intron_spanning
Chromosome Location:   13:103404334-103406786question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000003837

Gene Information

catalyzes the phosphorylation of Ser19 of nonmuscle myosin light chain; binds GTP-Cdc42 and plays a role in cytoskeleton organization [RGD, Feb 2006]

Gene Symbol:   Cdc42bpa
Gene Name:   Serine/threonine-protein kinase MRCK alpha
Aliases:   Not Available
RefSeq:   NM_053657
Ensembl:   ENSRNOG00000002841
Entrez:   114116
Chromosome Mapping:   13q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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