PrimePCR™ Probe Assay: Tpm1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Tpm1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tpm1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023178
Assay Design:   intron_spanning
Chromosome Location:   8:77159134-77161172question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000057641 ENSRNOT00000024617 ENSRNOT00000040808 ENSRNOT00000024575 ENSRNOT00000048044 ENSRNOT00000024493 ENSRNOT00000023567 ENSRNOT00000023605 ENSRNOT00000029076

Gene Information

This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Gene Symbol:   Tpm1
Gene Name:   Tropomyosin alpha-1 chain
Aliases:   Not Available
RefSeq:   NM_001034069 NM_001034070 NM_001034071 NM_001034073 NM_001034074 NM_001034072 NM_001034068 NM_019131 NM_001034075
Ensembl:   ENSRNOG00000018184
Entrez:   24851
Chromosome Mapping:   8q24

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