PrimePCR™ Probe Assay: Wt1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Wt1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Wt1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023936
Assay Design:   intron_spanning
Chromosome Location:   3:101773726-101796303question
Amplicon Length:   197
Splice Variants Targeted:   ENSRNOT00000067940

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008]

Gene Symbol:   Wt1
Gene Name:   Wilms tumor protein homolog
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000013074
Entrez:   24883
Chromosome Mapping:   3q32

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