PrimePCR™ Probe Assay: Masp1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024373
Assay Design:   intron_spanning
Chromosome Location:   11:79974855-79992597question
Amplicon Length:   75
Splice Variants Targeted:   ENSRNOT00000002507 ENSRNOT00000002512 ENSRNOT00000047678 ENSRNOT00000005175

Gene Information

serine protease that plays a role in the mannan-binding lectin complement pathway [RGD, Feb 2006]

Gene Symbol:   Masp1
Gene Name:   Mannan-binding lectin serine protease 1 Mannan-binding lectin serine protease 1 heavy chain Mannan-binding lectin serine protease 1 light chain
Aliases:   Not Available
RefSeq:   NM_022257
Ensembl:   ENSRNOG00000001827
Entrez:   64023
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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