PrimePCR™ Probe Assay: Mtdh, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $225.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024455
Assay Design:   intron_spanning
Chromosome Location:   7:72939773-72967003question
Amplicon Length:   181
Splice Variants Targeted:   ENSRNOT00000073507 ENSRNOT00000009989 ENSRNOT00000041065 ENSRNOT00000045470

Gene Information

human and mouse homologs are overexpressed in breast cancer tissue, bind to lung vasculature, and may play a role in promoting metastasis [RGD, Feb 2006]

Gene Symbol:   Mtdh
Gene Name:   Protein LYRIC
Aliases:   Not Available
RefSeq:   NM_133398
Ensembl:   ENSRNOG00000006870
Entrez:   170910
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.660000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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