PrimePCR™ Probe Assay: Vdr, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024473
Assay Design:   intron_spanning
Chromosome Location:   7:139554527-139563032question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000011601

Gene Information

binds vitamin D3; acts as a transcriptional regulator of the osteocalcin gene [RGD, Feb 2006]

Gene Symbol:   Vdr
Gene Name:   Vitamin D3 receptor
Aliases:   Not Available
RefSeq:   NM_017058
Ensembl:   ENSRNOG00000008574
Entrez:   24873
Chromosome Mapping:   7q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.120000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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