PrimePCR™ Probe Assay: Itga7, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Itga7, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Itga7, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024932
Assay Design:   intron_spanning
Chromosome Location:   7:3330873-3332445question
Amplicon Length:   143
Splice Variants Targeted:   ENSRNOT00000030453 ENSRNOT00000061657 ENSRNOT00000048267 ENSRNOT00000013022

Gene Information

alpha subunit of integrin alpha-7/beta-1; functions as a laminin receptor [RGD, Feb 2006]

Gene Symbol:   Itga7
Gene Name:   Integrin alpha-7 Integrin alpha-7 heavy chain Integrin alpha-7 light chain Integrin alpha-7 70 kDa form
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000007905
Entrez:   81008
Chromosome Mapping:   7q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.170000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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