PrimePCR™ Probe Assay: Hif3a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Hif3a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hif3a, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025640
Assay Design:   intron_spanning
Chromosome Location:   1:80233502-80236994question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000023490

Gene Information

binds HIF-responsive elements and activates transcription in vitro [RGD, Feb 2006]

Gene Symbol:   Hif3a
Gene Name:   Hypoxia-inducible factor 3-alpha
Aliases:   Not Available
RefSeq:   NM_022528
Ensembl:   ENSRNOG00000017198
Entrez:   64345
Chromosome Mapping:   1q21

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