PrimePCR™ Probe Assay: Pfkfb1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Pfkfb1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Pfkfb1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0027050
Assay Design:   intron_spanning
Chromosome Location:   X:23544710-23559613question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000033656

Gene Information

bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]

Gene Symbol:   Pfkfb1
Gene Name:   6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 6-phosphofructo-2-kinase Fructose-2,6-bisphosphatase
Aliases:   Not Available
RefSeq:   NM_012621
Ensembl:   ENSRNOG00000000165
Entrez:   24638
Chromosome Mapping:   Xq22-q31

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