PrimePCR™ Probe Assay: Agxt, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Agxt, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Agxt, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028810
Assay Design:   intron_spanning
Chromosome Location:   9:99945444-99948114question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000029127

Gene Information

This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013]

Gene Symbol:   Agxt
Gene Name:   Serine--pyruvate aminotransferase, mitochondrial
Aliases:   Not Available
RefSeq:   NM_030656
Ensembl:   ENSRNOG00000023856
Entrez:   24792
Chromosome Mapping:   9q34-q36

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