PrimePCR™ Probe Assay: Adarb1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Adarb1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Adarb1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028968
Assay Design:   intron_spanning
Chromosome Location:   20:14234401-14238198question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000001642

Gene Information

This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Adarb1
Gene Name:   Double-stranded RNA-specific editase 1 isoform 1
Aliases:   Not Available
RefSeq:   NM_012894 NM_001111055 NM_001111056 NM_001111057
Ensembl:   ENSRNOG00000001227
Entrez:   25367
Chromosome Mapping:   20p12

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