PrimePCR™ Probe Assay: Map2k1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0030333
Assay Design:   intron_spanning
Chromosome Location:   8:68841464-68846104question
Amplicon Length:   113
Splice Variants Targeted:   ENSRNOT00000013933

Gene Information

kinase that activates Mapk3 (Erk1) and Mapk1 (Erk2) kinases [RGD, Feb 2006]

Gene Symbol:   Map2k1
Gene Name:   Dual specificity mitogen-activated protein kinase kinase 1
Aliases:   Not Available
RefSeq:   NM_031643
Ensembl:   ENSRNOG00000010176
Entrez:   170851
Chromosome Mapping:   8q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.380000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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