PrimePCR™ Probe Assay: Tsc22d1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Tsc22d1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tsc22d1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0031135
Assay Design:   intron_spanning
Chromosome Location:   15:62252805-62347325question
Amplicon Length:   79
Splice Variants Targeted:   ENSRNOT00000060847 ENSRNOT00000058206 ENSRNOT00000001360 ENSRNOT00000048080 ENSRNOT00000025070

Gene Information

putative transcription regulating factor; upregulated by FSH in Sertoli cells [RGD, Feb 2006]

Gene Symbol:   Tsc22d1
Gene Name:   TSC22 domain family protein 1
Aliases:   Not Available
RefSeq:   NM_001109912 NM_013043
Ensembl:   ENSRNOG00000001030
Entrez:   498545
Chromosome Mapping:   15q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.660000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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