PrimePCR™ Probe Assay: Hnrnpab, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hnrnpab, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hnrnpab, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0037858
Assay Design:   intron_spanning
Chromosome Location:   10:36822217-36823418question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000004885 ENSRNOT00000033056 ENSRNOT00000065584 ENSRNOT00000018970 ENSRNOT00000020575 ENSRNOT00000047840 ENSRNOT00000003173 ENSRNOT00000003158

Gene Information

DNA binding protein; binds to Aldolase B promoter and may be involved in repression [RGD, Feb 2006]

Gene Symbol:   Hnrnpab
Gene Name:   CArG-binding factor A; Hnrpab protein; Protein Hnrnpab; RCG34663, isoform CRA_b; Type A/B hnRNP p38 (Heterogeneous nuclear ribonucleoprotein)
Aliases:   Not Available
RefSeq:   NM_031330
Ensembl:   ENSRNOG00000046272
Entrez:   83498
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.150000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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