PrimePCR™ Probe Assay: Trdn, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0047020
Assay Design:   Intron-spanning
Chromosome Location:   1:27115891-27177801question
Amplicon Length:   176
Splice Variants Targeted:   ENSRNOT00000055960 ENSRNOT00000042072 ENSRNOT00000067280

Gene Information

involved in calcium release process [RGD, Feb 2006]

Gene Symbol:   Trdn
Gene Name:   Triadin
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012609
Entrez:   59299
Chromosome Mapping:   1p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.010000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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