PrimePCR™ Probe Assay: UGT2B15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UGT2B15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UGT2B15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039976
Assay Design:   exonic
Chromosome Location:   4:69512911-69513025question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000551239 ENST00000549514 ENST00000547910 ENST00000305231 ENST00000508661 ENST00000446444 ENST00000335568 ENST00000511240 ENST00000512583 ENST00000305107 ENST00000381096 ENST00000503640 ENST00000512704 ENST00000514019 ENST00000286604 ENST00000457664 ENST00000317746 ENST00000338206 ENST00000265403 ENST00000458688 ENST00000251566 ENST00000420231

Gene Information

This gene encodes a member of the UDP-glycosyltransferase (UDPGT) family. The UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This protein displays activity towards several classes of xenobiotic substrates including simple phenolic compounds 7-hydroxylated coumarins flavonoids anthraquinones and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens. [provided by RefSeq Oct 2011]

Gene Symbol:   UGT2B15
Gene Name:   UDP glucuronosyltransferase 2 family, polypeptide B15
Aliases:   HLUG4, UDPGT 2B8, UDPGT2B15, UDPGTH3, UGT2B8
RefSeq:   NC_000004.11 NT_022778.16 NT_167250.1
Ensembl:   ENSG00000196620
Entrez:   7366
Chromosome Mapping:   4q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.930000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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