PrimePCR™ Probe Assay: NPM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NPM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040933
Assay Design:   exonic
Chromosome Location:   5:170819714-170819818question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000395068 ENST00000313269 ENST00000280562 ENST00000542749 ENST00000320201 ENST00000569564 ENST00000344147 ENST00000373714 ENST00000328969 ENST00000340957 ENST00000310585 ENST00000541296 ENST00000542457 ENST00000333577 ENST00000389561 ENST00000389562 ENST00000332482 ENST00000330386 ENST00000517671 ENST00000296930 ENST00000521672 ENST00000351986 ENST00000393820 ENST00000536681 ENST00000182377 ENST00000432730 ENST00000411624 ENST00000598359 ENST00000360565

Gene Information

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq Nov 2009]

Gene Symbol:   NPM1
Gene Name:   nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Aliases:   B23, MGC104254, NPM
RefSeq:   NC_000005.9 NT_023133.13 NG_016018.1
Ensembl:   ENSG00000181163
Entrez:   4869
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.460000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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