PrimePCR™ Probe Assay: TLE4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TLE4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TLE4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050432
Assay Design:   exonic
Chromosome Location:   9:82323038-82323138question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000376552 ENST00000376537 ENST00000265284 ENST00000428713 ENST00000490347 ENST00000467142 ENST00000496114 ENST00000417836 ENST00000376544 ENST00000376520 ENST00000376534

Gene Information

Description Not Available

Gene Symbol:   TLE4
Gene Name:   transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
Aliases:   BCE-1, BCE1, E(spI), ESG, ESG4, GRG4
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000106829
Entrez:   7091
Chromosome Mapping:   9q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.120000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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