PrimePCR™ Probe Assay: PTPN7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPN7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPN7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040794
Assay Design:   Intron-spanning
Chromosome Location:   1:202124691-202126917question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000007735 ENST00000576236 ENST00000323456 ENST00000579925 ENST00000457085 ENST00000355451 ENST00000378961 ENST00000308423 ENST00000591562 ENST00000264638 ENST00000308868 ENST00000380833 ENST00000380829 ENST00000421085 ENST00000441788 ENST00000373130 ENST00000360467 ENST00000527748 ENST00000331565 ENST00000450985 ENST00000286355 ENST00000377928 ENST00000341290 ENST00000433179 ENST00000379400 ENST00000379376 ENST00000373064 ENST00000367279 ENST00000309017 ENST00000477554 ENST00000477625 ENST

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   PTPN7
Gene Name:   protein tyrosine phosphatase, non-receptor type 7
Aliases:   BPTP-4, FLJ18703, HEPTP, LC-PTP, LPTP, PTPNI
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143851
Entrez:   5778
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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