PrimePCR™ Probe Assay: Camk2b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Camk2b, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Camk2b, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025361
Assay Design:   exonic
Chromosome Location:   14:86895520-86895614question
Amplicon Length:   65
Splice Variants Targeted:   ENSRNOT00000056937 ENSRNOT00000005441 ENSRNOT00000045935

Gene Information

beta subunit of calcium/calmodulin-dependent protein kinase II; may be involved in retinal development [RGD, Feb 2006]

Gene Symbol:   Camk2b
Gene Name:   Calcium/calmodulin-dependent protein kinase type II subunit beta isoform 2
Aliases:   Not Available
RefSeq:   NM_001042356 NM_001042354 NM_021739
Ensembl:   ENSRNOG00000004005
Entrez:   24245
Chromosome Mapping:   14q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.880000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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