PrimePCR™ Probe Assay: COL2A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL2A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL2A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023931
Assay Design:   Exonic
Chromosome Location:   12:48368003-48368478question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000380518 ENST00000337299 ENST00000395281

Gene Information

This gene encodes the alpha-1 chain of type II collagen a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis chondrodysplasia early onset familial osteoarthritis SED congenita Langer-Saldino achondrogenesis Kniest dysplasia Stickler syndrome type I and spondyloepimetaphyseal dysplasia Strudwick type. In addition defects in processing chondrocalcin a calcium binding protein that is the C-propeptide of this collagen molecule are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   COL2A1
Gene Name:   collagen, type II, alpha 1
Aliases:   ANFH, AOM, COL11A3, MGC131516, SEDC
RefSeq:   NC_000012.11 NG_008072.1 NT_029419.12
Ensembl:   ENSG00000139219
Entrez:   1280
Chromosome Mapping:   12q13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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