PrimePCR™ Probe Assay: RAPGEF3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAPGEF3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAPGEF3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024030
Assay Design:   Exonic
Chromosome Location:   12:48141515-48141619question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000541821 ENST00000395358 ENST00000397089 ENST00000405493 ENST00000171000 ENST00000389211 ENST00000548919 ENST00000389212 ENST00000449771 ENST00000549151

Gene Information

Description Not Available

Gene Symbol:   RAPGEF3
Gene Name:   Rap guanine nucleotide exchange factor (GEF) 3
Aliases:   CAMP-GEFI, EPAC, EPAC1, HSU79275, MGC21410, bcm910
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000079337
Entrez:   10411
Chromosome Mapping:   12q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.910000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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