PrimePCR™ Probe Assay: HLA-DOB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-DOB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DOB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040215
Assay Design:   exonic
Chromosome Location:   6:32782945-32783079question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000426644 ENST00000453971 ENST00000420764 ENST00000395550 ENST00000383733 ENST00000337000 ENST00000455886 ENST00000358747 ENST00000564819 ENST00000544589 ENST00000454286 ENST00000413252 ENST00000454969 ENST00000448690 ENST00000447178 ENST00000439310 ENST00000456406 ENST00000455155 ENST00000527042 ENST00000366663 ENST00000470540 ENST00000427697 ENST00000523410 ENST00000438763 ENST00000447394

Gene Information

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB) both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DOB
Gene Name:   major histocompatibility complex, class II, DO beta
Aliases:   DOB, FLJ57033
RefSeq:   NC_000006.11 NG_012008.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167249.1
Ensembl:   ENSG00000241106
Entrez:   3112
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.040000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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