PrimePCR™ Probe Assay: TNFRSF6B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNFRSF6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFRSF6B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040231
Assay Design:   exonic
Chromosome Location:   20:62328203-62328428question
Amplicon Length:   196
Splice Variants Targeted:   ENST00000528154 ENST00000392557 ENST00000379937 ENST00000546474 ENST00000446501 ENST00000371655 ENST00000318100 ENST00000310430 ENST00000273783 ENST00000444495 ENST00000369996 ENST00000445265 ENST00000323668 ENST00000439160 ENST00000394269 ENST00000427724 ENST00000504761 ENST00000513346 ENST00000515516 ENST00000451292 ENST00000377797 ENST00000245663 ENST00000395104 ENST00000337137 ENST00000291900 ENST00000580310 ENST00000403729 ENST00000334456 ENST00000418754 ENST00000542167 ENST00000397841 ENST

Gene Information

This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene which encodes regulator of telomere elongation helicase 1 (RTEL1) generates a non-coding transcript. [provided by RefSeq Feb 2011]

Gene Symbol:   TNFRSF6B
Gene Name:   tumor necrosis factor receptor superfamily, member 6b, decoy
Aliases:   DCR3, DJ583P15.1.1, M68, TR6
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000243509
Entrez:   8771
Chromosome Mapping:   20q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.110000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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