PrimePCR™ Probe Assay: BAX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BAX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BAX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040666
Assay Design:   exonic
Chromosome Location:   19:49464074-49464174question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000358337 ENST00000391871 ENST00000539787 ENST00000293288 ENST00000506183 ENST00000354470 ENST00000415969 ENST00000345358 ENST00000258436 ENST00000296452 ENST00000412950 ENST00000459879 ENST00000255108

Gene Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2 and functions as an apoptotic activator. This protein is reported to interact with and increase the opening of the mitochondrial voltage-dependent anion channel (VDAC) which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants which encode different isoforms have been reported for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   BAX
Gene Name:   BCL2-associated X protein
Aliases:   BCL2L4
RefSeq:   NC_000019.9 NG_008152.1 NT_011109.16 NG_012191.1
Ensembl:   ENSG00000087088
Entrez:   581
Chromosome Mapping:   19q13.3-q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.990000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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