PrimePCR™ Probe Assay: AKT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040687
Assay Design:   exonic
Chromosome Location:   19:40762883-40771151question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000392038 ENST00000311278 ENST00000579047 ENST00000441941 ENST00000486368 ENST00000596634 ENST00000452077 ENST00000392037 ENST00000416362 ENST00000578123 ENST00000423127 ENST00000358335 ENST00000456441 ENST00000583859 ENST00000580747 ENST00000416994 ENST00000427375 ENST00000424901 ENST00000555926 ENST00000555380 ENST00000554848 ENST00000555528 ENST00000402615 ENST00000349310 ENST00000407796 ENST00000554581

Gene Information

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   AKT2
Gene Name:   v-akt murine thymoma viral oncogene homolog 2
Aliases:   PKBB, PKBBETA, PRKBB, RAC-BETA
RefSeq:   NC_000019.9 NG_012038.1 NT_011109.16
Ensembl:   ENSG00000105221
Entrez:   208
Chromosome Mapping:   19q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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