PrimePCR™ Probe Assay: NME2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NME2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NME2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041359
Assay Design:   exonic
Chromosome Location:   17:49248887-49249008question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000372442 ENST00000562955 ENST00000354905 ENST00000511828 ENST00000512816 ENST00000393193 ENST00000376392 ENST00000555572 ENST00000393198 ENST00000393185 ENST00000393183 ENST00000514264 ENST00000513177 ENST00000512737 ENST00000503064 ENST00000393190 ENST00000264018 ENST00000372743 ENST00000372741 ENST00000343694 ENST00000419308 ENST00000377115 ENST00000319993 ENST00000367119 ENST00000524892 ENST00000298815 ENST00000368316 ENST00000456909 ENST00000295641 ENST00000580607 ENST00000596625

Gene Information

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq Nov 2010]

Gene Symbol:   NME2
Gene Name:   non-metastatic cells 2, protein (NM23B) expressed in
Aliases:   MGC111212, MGC2212, NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000011052
Entrez:   4831
Chromosome Mapping:   17q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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