PrimePCR™ Probe Assay: SMC1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SMC1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMC1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049584
Assay Design:   exonic
Chromosome Location:   X:53440376-53441819question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000322213 ENST00000428014 ENST00000375340 ENST00000594240 ENST00000599303 ENST00000594131

Gene Information

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis although those complexes at the kinetochore remain. Therefore the encoded protein is thought to be an important part of functional kinetochores. In addition this protein interacts with BRCA1 and is phosphorylated by ATM indicating a potential role for this protein in DNA repair. This gene which belongs to the SMC gene family is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq Jul 2008]

Gene Symbol:   SMC1A
Gene Name:   structural maintenance of chromosomes 1A
Aliases:   CDLS2, DKFZp686L19178, DXS423E, KIAA0178, MGC138332, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB
RefSeq:   NC_000023.10 NG_006988.1 NT_011630.14
Ensembl:   ENSG00000072501
Entrez:   8243
Chromosome Mapping:   Xp11.22-p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.690000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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