PrimePCR™ Probe Assay: MAP2K7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP2K7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP2K7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049651
Assay Design:   exonic
Chromosome Location:   19:7977999-7978096question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000397981 ENST00000397983 ENST00000397979 ENST00000545011

Gene Information

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2 and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1 MAP3K2/MEKK2MAP3K3/MEKK5 and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found but only one transcript variant has been supported and defined. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP2K7
Gene Name:   mitogen-activated protein kinase kinase 7
Aliases:   Jnkk2, MAPKK7, MKK7, PRKMK7
RefSeq:   NC_000019.9 NT_077812.2
Ensembl:   ENSG00000076984
Entrez:   5609
Chromosome Mapping:   19p13.3-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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