PrimePCR™ Probe Assay: CCT4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCT4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050879
Assay Design:   exonic
Chromosome Location:   2:62099284-62099432question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000394440 ENST00000544079 ENST00000538252 ENST00000544185

Gene Information

The chaperonin containing TCP1 (MIM 186980) complex (CCT) also called the TCP1 ring complex consists of 2 back-to-back rings each containing 8 unique but homologous subunits such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130) as well as alpha-transducin (MIM 139330) (Won et al. 1998 [PubMed 9819444]).[supplied by OMIM Mar 2008]

Gene Symbol:   CCT4
Gene Name:   chaperonin containing TCP1, subunit 4 (delta)
Aliases:   CCT-DELTA, Cctd, MGC126164, MGC126165, SRB
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115484
Entrez:   10575
Chromosome Mapping:   2p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.610000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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