PrimePCR™ Probe Assay: VASP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VASP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VASP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051360
Assay Design:   exonic
Chromosome Location:   19:46010869-46010983question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000409709 ENST00000458637 ENST00000409619 ENST00000458169 ENST00000582783 ENST00000392035 ENST00000357877 ENST00000356897 ENST00000490531 ENST00000449001 ENST00000374088 ENST00000374075 ENST00000312033 ENST00000307564 ENST00000282059 ENST00000321319 ENST00000524669 ENST00000262244 ENST00000367742 ENST00000338920 ENST00000392078 ENST00000426496 ENST00000382034 ENST00000536370 ENST00000397224 ENST00000216187 ENST00000397223 ENST00000328771 ENST00000403750 ENST00000227756 ENST00000315005 ENST

Gene Information

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq Jul 2008]

Gene Symbol:   VASP
Gene Name:   vasodilator-stimulated phosphoprotein
Aliases:   Not Available
RefSeq:   NC_000019.9 NG_013332.1 NT_011109.16
Ensembl:   ENSG00000125753
Entrez:   7408
Chromosome Mapping:   19q13.2-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.000000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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