PrimePCR™ Probe Assay: IFNAR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: IFNAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IFNAR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052311
Assay Design:   exonic
Chromosome Location:   21:34707854-34707953question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000270139 ENST00000442071 ENST00000442357 ENST00000416947

Gene Information

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases which in turn phosphorylate several proteins including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq Jul 2008]

Gene Symbol:   IFNAR1
Gene Name:   interferon (alpha, beta and omega) receptor 1
Aliases:   AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000142166
Entrez:   3454
Chromosome Mapping:   21q22.1,21q22.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.860000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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