PrimePCR™ Probe Assay: TNFRSF4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TNFRSF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFRSF4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055125
Assay Design:   exonic
Chromosome Location:   1:1147359-1147487question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000598504 ENST00000544886 ENST00000373409 ENST00000499247 ENST00000268957 ENST00000259053 ENST00000504830 ENST00000352040 ENST00000379236 ENST00000311127 ENST00000458420 ENST00000261381

Gene Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFRSF4
Gene Name:   tumor necrosis factor receptor superfamily, member 4
Aliases:   ACT35, CD134, OX40, TXGP1L
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000186827
Entrez:   7293
Chromosome Mapping:   1p36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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