PrimePCR™ Probe Assay: MAPT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAPT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAPT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055132
Assay Design:   exonic
Chromosome Location:   17:44073875-44074014question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000549053 ENST00000550769 ENST00000447599 ENST00000549598 ENST00000550493 ENST00000553101 ENST00000546644 ENST00000547239 ENST00000552530 ENST00000552974 ENST00000308595 ENST00000334239 ENST00000446361 ENST00000420682 ENST00000415613 ENST00000571987 ENST00000574436 ENST00000431008 ENST00000576518 ENST00000344290 ENST00000262410 ENST00000351559 ENST00000340799 ENST00000535772 ENST00000347967

Gene Information

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex regulated alternative splicing giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease Pick's disease frontotemporal dementia cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPT
Gene Name:   microtubule-associated protein tau
Aliases:   DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
RefSeq:   NC_000017.10 NT_010783.15 NT_167251.1 NG_007398.1
Ensembl:   ENSG00000186868
Entrez:   4137
Chromosome Mapping:   17q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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