PrimePCR™ Probe Assay: IL2RG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL2RG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL2RG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058342
Assay Design:   exonic
Chromosome Location:   X:70330752-70330844question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000374202 ENST00000464642 ENST00000487883 ENST00000473378

Gene Information

The protein encoded by this gene is an important signaling component of many interleukin receptors including those of interleukin -2 -4 -7 and -21 and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID) as well as X-linked combined immunodeficiency (XCID) a less severe immunodeficiency disorder. [provided by RefSeq Mar 2010]

Gene Symbol:   IL2RG
Gene Name:   interleukin 2 receptor, gamma
Aliases:   CD132, CIDX, IL-2RG, IMD4, P64, SCIDX, SCIDX1
RefSeq:   NC_000023.10 NG_009088.1 NG_021141.1 NT_011669.17
Ensembl:   ENSG00000147168
Entrez:   3561
Chromosome Mapping:   Xq13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.330000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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