PrimePCR™ Probe Assay: FCER1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FCER1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FCER1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0025956
Assay Design:   Intron-spanning
Chromosome Location:   1:159275968-159277601question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000368114 ENST00000368115

Gene Information

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit a beta subunit and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq Aug 2011]

Gene Symbol:   FCER1A
Gene Name:   Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
Aliases:   FCE1A, FcERI
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000179639
Entrez:   2205
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.900000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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