PrimePCR™ Probe Assay: PDGFRA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDGFRA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDGFRA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026381
Assay Design:   Intron-spanning
Chromosome Location:   4:55124944-55127353question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000257290 ENST00000508170 ENST00000512143 ENST00000503856 ENST00000504461 ENST00000512522

Gene Information

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development wound healing and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome somatic and familial gastrointestinal stromal tumors and a variety of other cancers. [provided by RefSeq Mar 2012]

Gene Symbol:   PDGFRA
Gene Name:   platelet-derived growth factor receptor, alpha polypeptide
Aliases:   CD140A, MGC74795, PDGFR2, RHEPDGFRA
RefSeq:   NC_000004.11 NG_009250.1 NT_022853.15
Ensembl:   ENSG00000134853
Entrez:   5156
Chromosome Mapping:   4q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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