PrimePCR™ Probe Assay: ARHGAP32, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARHGAP32, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGAP32, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026458
Assay Design:   Intron-spanning
Chromosome Location:   11:128856473-128857998question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000310343 ENST00000392657 ENST00000524655 ENST00000527272 ENST00000356092 ENST00000457677

Gene Information

RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al. 2003 [PubMed 12531901]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP32
Gene Name:   Rho GTPase activating protein 32
Aliases:   GC-GAP, GRIT, KIAA0712, MGC1892, PX-RICS, RICS, p200RhoGAP, p250GAP
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000134909
Entrez:   9743
Chromosome Mapping:   11q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.470000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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