PrimePCR™ Probe Assay: MCM6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MCM6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MCM6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027301
Assay Design:   Intron-spanning
Chromosome Location:   2:136605693-136609059question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000264156

Gene Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2 4 and 7 proteins possesses DNA helicase activity and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity suggesting a role in the regulation of DNA replication. [provided by RefSeq Jul 2008]

Gene Symbol:   MCM6
Gene Name:   minichromosome maintenance complex component 6
Aliases:   MCG40308, Mis5, P105MCM
RefSeq:   NC_000002.11 NG_008104.1 NG_008958.1 NT_022135.16
Ensembl:   ENSG00000076003
Entrez:   4175
Chromosome Mapping:   2q21

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