PrimePCR™ Probe Assay: NRG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NRG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NRG1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027789
Assay Design:   Intron-spanning
Chromosome Location:   8:32611890-32613972question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000518104 ENST00000519301 ENST00000523534 ENST00000523079 ENST00000287842 ENST00000405005 ENST00000521670 ENST00000522402 ENST00000519240 ENST00000338921 ENST00000356819 ENST00000287840 ENST00000287845 ENST00000341377 ENST00000539990

Gene Information

The protein encoded by this gene was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. This protein is a signaling protein that mediates cell-cell interactions and plays critical roles in the growth and development of multiple organ systems. It is known that an extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are tissue-specifically expressed and differ significantly in their structure and thereby these isoforms are classified into types I II III IV V and VI. The gene dysregulation has been linked to diseases such as cancer schizophrenia and bipolar disorder (BPD). [provided by RefSeq May 2009]

Gene Symbol:   NRG1
Gene Name:   neuregulin 1
Aliases:   ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, NDF, SMDF
RefSeq:   NC_000008.10 NT_167187.1 NG_012005.1
Ensembl:   ENSG00000157168
Entrez:   3084
Chromosome Mapping:   8p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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