PrimePCR™ Probe Assay: MUC5B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MUC5B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MUC5B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028135
Assay Design:   Intron-spanning
Chromosome Location:   11:1279560-1280989question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000529681 ENST00000526859 ENST00000406844 ENST00000447027 ENST00000349637 ENST00000546052

Gene Information

This gene encodes a member of the mucin family of proteins which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases including sinus mucosa of chronic rhinosinusitis (CRS) CRS with nasal polyposis chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease and it may be involved in the pathogenesis of these diseases. [provided by RefSeq Jul 2010]

Gene Symbol:   MUC5B
Gene Name:   mucin 5B, oligomeric mucus/gel-forming
Aliases:   MG1, MUC-5B, MUC5, MUC9
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000117983
Entrez:   727897
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.988300
y-intercept 35.230000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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