PrimePCR™ Probe Assay: SERPINA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SERPINA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SERPINA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028950
Assay Design:   Intron-spanning
Chromosome Location:   14:94847342-94848978question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000440909 ENST00000393087 ENST00000393088 ENST00000404814 ENST00000449399 ENST00000402629 ENST00000448921 ENST00000437397 ENST00000355814

Gene Information

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase plasmin thrombin trypsin chymotrypsin and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SERPINA1
Gene Name:   serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Aliases:   A1A, A1AT, AAT, MGC23330, MGC9222, PI, PI1, PRO2275, alpha1AT
RefSeq:   NC_000014.8 NT_026437.12 NG_008290.1
Ensembl:   ENSG00000197249
Entrez:   5265
Chromosome Mapping:   14q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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