PrimePCR™ Probe Assay: SNAP91, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SNAP91, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNAP91, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040616
Assay Design:   Intron-spanning
Chromosome Location:   6:84372081-84417524question
Amplicon Length:   181
Splice Variants Targeted:   ENST00000521485 ENST00000369694 ENST00000195649 ENST00000439399 ENST00000521743 ENST00000520302 ENST00000520213 ENST00000521931 ENST00000519779 ENST00000518309 ENST00000369690 ENST00000523484 ENST00000428679 ENST00000437520

Gene Information

Description Not Available

Gene Symbol:   SNAP91
Gene Name:   synaptosomal-associated protein, 91kDa homolog (mouse)
Aliases:   AP180, CALM, DKFZp781O0519, KIAA0656
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000065609
Entrez:   9892
Chromosome Mapping:   6q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.810000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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