PrimePCR™ Probe Assay: Ifna2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Ifna2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ifna2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062575
Assay Design:   exonic
Chromosome Location:   4:88683528-88683669question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000047786 ENSMUST00000160844 ENSMUST00000160800 ENSMUST00000159778 ENSMUST00000174337 ENSMUST00000172466 ENSMUST00000054059 ENSMUST00000161402 ENSMUST00000174331 ENSMUST00000072671 ENSMUST00000176174 ENSMUST00000084731 ENSMUST00000115048 ENSMUST00000115046 ENSMUST00000102895 ENSMUST00000087400 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000112931 ENSMUST00000112930 ENSMUST00000130732 ENSMUST00000113876 ENSMUST00000113883 ENSMUST00000113882 ENSMUST00000113878 ENSMUST00000093942 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ifna2
Gene Name:   Interferon alpha 2
Aliases:   Ifa2
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000078354
Entrez:   15965
Chromosome Mapping:   4 C4|4 42.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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