PrimePCR™ Probe Assay: Gstm1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024108
Assay Design:   intron_spanning
Chromosome Location:   2:230275449-230276748question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000047139

Gene Information

multifunctional enzyme thought to have scavenger function; able to neutralize harmful metabolites from foreign substances [RGD, Feb 2006]

Gene Symbol:   Gstm1
Gene Name:   Glutathione S-transferase Mu 1
Aliases:   Not Available
RefSeq:   NM_017014
Ensembl:   ENSRNOG00000029726
Entrez:   24423
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.680000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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