PrimePCR™ Probe Assay: Cacna1c, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Cacna1c, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cacna1c, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025802
Assay Design:   intron_spanning
Chromosome Location:   4:217039705-217184708question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000074194 ENSRNOT00000052017 ENSRNOT00000041571 ENSRNOT00000009268 ENSRNOT00000051846

Gene Information

subunit of an L-type calcium channel that may be involved in bone metabolism and compensatory renal growth [RGD, Feb 2006]

Gene Symbol:   Cacna1c
Gene Name:   Voltage-dependent L-type calcium channel subunit alpha-1C
Aliases:   Not Available
RefSeq:   NM_012517
Ensembl:   ENSRNOG00000007090
Entrez:   24239
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.080000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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