PrimePCR™ Probe Assay: LPAR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LPAR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LPAR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057514
Assay Design:   exonic
Chromosome Location:   19:19737882-19738029question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000374429 ENST00000395793 ENST00000216129 ENST00000358894 ENST00000422202 ENST00000326996 ENST00000435874 ENST00000398897 ENST00000398911 ENST00000417133 ENST00000398910 ENST00000398919 ENST00000442448 ENST00000394809 ENST00000536476 ENST00000588578 ENST00000338270 ENST00000285208 ENST00000543906 ENST00000586703 ENST00000407877 ENST00000542587 ENST00000591042 ENST00000588233 ENST00000402866 ENST00000395209 ENST00000538510 ENST00000541709 ENST00000219406 ENST00000404312 ENST00000246657 ENST

Gene Information

This gene encodes a member of family I of the G protein-coupled receptors as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization a critical cellular response to LPA in cells through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LPAR2
Gene Name:   lysophosphatidic acid receptor 2
Aliases:   EDG-4, EDG4, FLJ93869, LPA-2, LPA2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000064547
Entrez:   9170
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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