PrimePCR™ Probe Assay: SLC25A20, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027640
Assay Design:   Intron-spanning
Chromosome Location:   3:48929465-48936178question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000430379 ENST00000319017 ENST00000544097

Gene Information

This gene product is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency which can cause a variety of pathological conditions such as hypoglycemia cardiac arrest hepatomegaly hepatic dysfunction and muscle weakness and is usually lethal in new born and infants. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC25A20
Gene Name:   solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
Aliases:   CAC, CACT
RefSeq:   NC_000003.11 NG_008171.1 NT_022517.18
Ensembl:   ENSG00000178537
Entrez:   788
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.380000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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