PrimePCR™ Probe Assay: CD44, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CD44, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD44, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028986
Assay Design:   Intron-spanning
Chromosome Location:   11:35201821-35208430question
Amplicon Length:   156
Splice Variants Targeted:   ENST00000415148 ENST00000428726 ENST00000433892 ENST00000434472 ENST00000352818 ENST00000442151 ENST00000525211 ENST00000526000 ENST00000279452 ENST00000527889 ENST00000528455 ENST00000531873 ENST00000433354 ENST00000449691 ENST00000437706 ENST00000360158 ENST00000263398

Gene Information

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands such as osteopontin collagens and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation recirculation and homing hematopoiesis and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms however the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein and may be related to tumor metastasis. [provided by RefSeq Jul 2008]

Gene Symbol:   CD44
Gene Name:   CD44 molecule (Indian blood group)
Aliases:   CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, Pgp1
RefSeq:   NC_000011.9 NT_009237.18 NG_008937.1
Ensembl:   ENSG00000026508
Entrez:   960
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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